I remember my first battles with protein purification—they were long, strenuous, and I never won. I couldn’t even get a few milligrams of pure, functioning protein. It became my least favorite lab activity. I began to cringe every time I heard the words “p53 tumor suppressor protein,” for it reminded me of my never-ending failures associated with protein purification.
This experience characterized most of my first full-time undergraduate summer research experience. It left me with a sour perspective toward research, because spending five weeks on failed purifications caused me to get no real work done on my actual project. I couldn’t start my project until I had pure, functioning protein. I never did get the protein I needed, so I finished up my final presentation for my summer program in agony.
I know what I’m saying right now must sound melancholy, but I assure you it will get better. In research, you will often be plagued with situations in which your experiments/procedures do not work for weeks or months on end. However, this is all for good reason, because it is how you learn.
Recently, in my second undergraduate research experience, I have come back to protein purification. I carried out four purifications over my first two weeks, and I have never been more successful. On one of my purifications, I obtained 22 mg of pure protein. This may not sound like much, but in the world of proteins it is quite a bit.
My first experiences taught me that you should never let your first pass on any subject—school or research—define how you feel about it for the rest of your life. It takes training and perseverance to become good at something, so you should always be willing to give it another try before you decide it’s not for you. Just like how protein purification became easier and more successful for me in my second experience, chances are, you will find the same successes in your own endeavors.