My War with Protein Purification

I remember my first battles with protein purification—they were long, strenuous, and I never won. I couldn’t even get a few milligrams of pure, functioning protein. It became my least favorite lab activity. I began to cringe every time I heard the words “p53 tumor suppressor protein,” for it reminded me of my never-ending failures associated with protein purification.

 

This experience characterized most of my first full-time undergraduate summer research experience. It left me with a sour perspective toward research, because spending five weeks on failed purifications caused me to get no real work done on my actual project. I couldn’t start my project until I had pure, functioning protein. I never did get the protein I needed, so I finished up my final presentation for my summer program in agony.

 

I know what I’m saying right now must sound melancholy, but I assure you it will get better. In research, you will often be plagued with situations in which your experiments/procedures do not work for weeks or months on end. However, this is all for good reason, because it is how you learn.

 

Recently, in my second undergraduate research experience, I have come back to protein purification. I carried out four purifications over my first two weeks, and I have never been more successful. On one of my purifications, I obtained 22 mg of pure protein. This may not sound like much, but in the world of proteins it is quite a bit.

 

My first experiences taught me that you should never let your first pass on any subject—school or research—define how you feel about it for the rest of your life. It takes training and perseverance to become good at something, so you should always be willing to give it another try before you decide it’s not for you. Just like how protein purification became easier and more successful for me in my second experience, chances are, you will find the same successes in your own endeavors.

The End of a Run

Wow! As this summer program comes to an end, I am exhausted…

Not the overwhelmingly sleep-deprived exhaustion. But instead, the kind of exhaustion that shows that you have accomplished something—like being out of breath after running.

At the end of a run, you know that you have earned not only the tiredness and soreness, but also the strength that ensues. So as I gear up to sprint the last couple of weeks of the MARC summer program, I know that I will be even more exhausted than I am now. But I also know that there is a great reward waiting for me on the other side of the finish line. I have struggled in workshops to improve my writing and presenting and labored in lab to develop my technique and skills. But there is satisfaction in knowing that you have challenged yourself, struggling through new obstacles and emerging stronger and wiser than before. There is fulfillment that comes with presenting work that you have invested time in and believe in. And there is gratification that comes with knowing you have worked on research that has helped not only your growth, but could also potentially benefit many others.

However, as I run my course, I am not alone. I have met second years that hold so much promise, my fellow third years that have offered encouragement as we run similar courses, and fourth years that are looking ahead to bright futures. As we run along side one another, my peers have seen me struggle when my stride faltered but have only given me positive support to continue. They have provided me with laughs to distract from my labored breathing telling me to slow. To sum it up, they have been pretty awesome!

So if you are looking at the CSEP programs and wondering if all the hard work is worth it… it is. The opportunities that these programs give you are so unique, and only a small group of undergrads gets to experience them each year. From workshops on conferences and graduate school to dinners with experienced scientists from academia and industry, there is always something new to learn, and there is always someone new to meet. So, if you are like me, you may be exhausted, but the soreness just means that you are getting stronger.

Research. What is it good for?

A few nights ago, my fellow CSEP scholars and I had dinner with faculty from STEM fields all across the UCSB campus, and we chatted about this very question: what is research good for? From these conversations, I got a lot of insight from people extremely well-qualified to answer such a question. So I am here to share this expert advice so that you may learn these lessons sooner than I did.

There is the more obvious answer to what the benefit of research is: resumé booster. Whatever it is that you want to do in STEM, research experience is helpful. Whether you want to go on to get your Masters, go to graduate school, or go straight into industry after your undergraduate education, previous lab experience, in my opinion, is an unspoken requirement for admission. This was reinforced by a conversation I had with a professor at dinner. He told me that research experience is often used as a filter to narrow down the pool of applicants. Therefore, many smart and capable candidates are not even given a chance—a sad reality. However, this is not done in malice. It is done because the amount of qualified candidates is so large (and growing every year) that they must narrow them down somehow. Now, I am not telling you this story to scare you or to say that those without research experience have no chance of progressing in their careers. I am telling you this as advice to seize all of the opportunities you can so that you are not tossed aside when you really are a smart and capable candidate. Therefore, research in your undergraduate career gives you a leg up, which is nothing but helpful in the competitive world we live in. Many of the skills and techniques that you need in both grad school and industry are taught through lab experience as an undergraduate, such as how to set up, purify, and analyze reactions. You learn what the research process is like and how to move forward when your reactions either do or do not work. It even teaches you what lab culture is like and how to navigate within it. This, among many other reasons, is why previous lab experience makes you a more desirable candidate.

While this may be a good enough reason to jump on board the undergraduate research train, it is not the only or most important answer to why research is good. Research (at all levels) teaches you about yourself. Personally, it has taught me what my passion is and, as a result, what I want to do with my future. It has forced me to carry on in the face of adversity, whether that adversity came from a project failing or from lacking role models for my education. From the people I have met and the experiences I have had, I strongly believe that research helps produce people that are driven and strong, as well as guides them to careers they love. At dinner, Professor Joel Rothman told me that he loved his job and would not want another one, and I believed every word. Not only because of the conviction in his voice but also because of his story. He had taken time off during graduate school and found his way into winemaking. After a few years, he had made it to the top of the winemaking industry. However, once there, he looked around and saw what his future would be like, and he saw a life that could not give him the excitement and gratification that research could. So he went back to graduate school, and although he did not take the typical path to get to where he is, Professor Joel Rothman is now a successful professor, mentor, and researcher. Therefore, I would like to leave you with this remark: while research makes future employers happy, it should, first and foremost, make you happy.

Spirited Away (Microbiology edition)

My first experience with undergraduate research has been like one of my favorite movies, Spirited Away. Like the main character Chihiro, I’ve entered into a dreamlike world with fantastic things I’ve never seen before. My parents did not transform into pigs and abandon me like Chihiro’s so I’m not nearly as scared as she was, but I, like Chihiro, initially felt nervous and a little lost.

I entered the spirit world (lab) not knowing a lot about spirits (microbiology, bacterial genetics, lab techniques) and I needed a mentor. Luckily, a post-doc, Zach Ruhe, agreed to guide me. I like to think of Zach as Kamaji, the bath-house boiler man/spider. He has six arms that are always busy in his work, yet he still has given me the chance to work and learn in the lab. I’ve already learned so much from my mentor–how to present ideas, how to express myself in technical writing, how to handle lab skillz with finesse, how to work harder than you think you should, how to play Black & Yellow on Google talk–and I am greatly appreciative.

I also have other helpers in the lab. August, a UCSB 2015 graduate, is a noble role model like Haku. He checks in on me, gives me good advice, and kindly offers zucchini bread snacks to me. Interestingly, Haku and August are both spirits controlled by tyrannical forces: Haku’s being Yubaba, August’s being the medical school application process. My other helper is Jing, a lab technician. I liken Jing to Lin, a sister-like co-worker who comes in from time to time to help out Chihiro and guide her in her journey.

This journey has just begun, but I am beginning to gain more confidence working in the spirit world. The coming-of-age theme of Spirited Away definitely resonates with me through this experience as I’m gaining more responsibility and starting to become a “real” scientist. I’m excited to continue working on our brand new project about antibiotic resistance and I am eager to report back when we’ve gotten more data. Thanks for reading.

-B

Here’s a picture of me slaving away at the lab:

Bianca in the lab