The Secret Life of Worms

I never knew that worms could be so intricate. You’d think that a worm would be so simple, just eating until its life is complete but surely I have been very surprised by how much there is to know about them. I never thought I would be so fond of something so small. When I see a whole bunch of little squiggly lines on a plate, I know that my worms are at the peak of their growth. You see, the plate is where the worms live and lines of bacteria drawn on the plate are their food source. When they run out of food, they lay all of their eggs. They instinctively do this in order to keep their species going-perfect example of survival of the fittest.

They are stubborn little animals I might add. I’m currently trying to perfect the technique of synchronization. Synchronization is the process of treating a set of worms with bleach and sodiumhydroxide. Bleach dissolves every stage of worm except for the embryos (as long as the embryos aren’t left in bleach for too long). The purpose of this is to obtain a synchronized population of worms, each worm being in the exact same developmental stage as those around it. However, despite treating the worms with bleach for long enough time periods for them to dissolve, I still find quite a few worms that have survived this.

On a good day, if I focus hard enough, I can see the slight movement of the worms with my naked eye. All the other days I spend my time looking at them through a microscope. Although completing my research project this summer is a great priority for me, my greatest goal is to see an embryo develop into a small, active worm. I haven’t been able to witness this yet. If I might be quite honest, this might be a larger task than I anticipated. This would require me to stare at a specific embryo for about ten hours and have a little bit of luck.

Update at 6 weeks in the lab.

Throughout the Gorman Scholars program, I have learned a lot of things. There are two that stand out from the rest.

#1. The program has made me realize how much I want to pursue graduate school. Before I was a little unsure about going to graduate school because I barely knew anything about it. But this program has helped me see it for what it is and the graduate student panel was really helpful. It really helped me start thinking and actually planning for graduate school.

#2. The program has made me realize how much I like the lab setting. I have been struggling to pick between a hospital setting or a lab setting for my future career and now that I have been in a lab for about 6 weeks, the decision between the two has become very easy. I like how quiet and flexible it is in the lab. I am a person that does not do very well in an environment that is busy and filled with people. I hope to continue in this lab onto next year because I have been really enjoying it. For next summer, I really want to travel abroad and do a research internship in another country.

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Now that it has been six weeks in the lab, my last post seems a little funny to read. I could tell from my writing that I was struggling in the lab and this is still the case but it has gotten so much better. I am becoming more and more independent. I have been starting to get the hang of things pretty quickly while three weeks ago, it took me a long time. Before, when I came into the lab, I used to see it as something I had to do… but recently I felt myself wanting to come in… I was genuinely excited to go in and do stuff. I hope this genuine interest keeps leading me in this research lab.

Perks of AIM

One of the great perks of this program is the weekly career development seminars and skills workshops. My personal favorites were the seminars by Professor Schow and Professor Valentine as well as the graduate school panel.

It was very interesting to hear about about Professor Schow’s experience in industry as a researcher versus in an academic institution, as well as how he ended up choosing between schools and different careers. And even though I didn’t understand all of the presentation, Professor Schow gave a great overview of his field and research. For Professor Valentine’s talk, I really enjoyed her emphasis on how random and nonlinear the future could be. She stressed the importance of creating opportunities and asking questions. Overall, it was pretty encouraging to hear that professors too had times in their lives where they weren’t sure or couldn’t choose, especially as I approach the end of my undergraduate education.

The graduate school panel was super helpful in that I got a better sense of what graduate school life is like and there was a ton of great advice. The panel emphasized the importance of flexibility and time management. In addition, they stressed the importance of choosing a good advisor and a healthy lab environment. Being able to ask 6 STEM students about graduate school was a very helpful and eye opening experience!

Overall, my favorite part about all these seminars and panels is learning about the many different ways people ended up at UCSB. Although it’s obvious, it’s always reassuring to remember that there are so many ways to go about one’s career. These seminars and panels also serve as a great reminder that life does not happen 100% according to plan or formula; there are failures and huge moments of uncertainty, along with great opportunities to learn and meet wonderful people.

Why am I so dumb?

Why am I so dumb?

This thought was ringing in my head repeatedly throughout all of first week of research… and I have to say it was draining and it made me not even want to do anything. I felt like I was so lost, useless, and unfit to be in a lab. I could not even follow simple directions that were written on paper or even do simple math calculations because that thought had consumed and drained my mind that I actually started acting like it. I think it was also fear and nerves that got to me. I was afraid of messing up that I messed up even more.

I still feel like this from time to time but not as much. But I always tell myself that this is typical of starting anything new. I am going to mess up and mess up again but I know I will be better at it the third time around. It’s important to keep this in mind if you are going to be in a lab soon. For me, it just takes a little bit of time to get used to everything and now at the end of the third week… I feel much better adjusted and feel like I am truly contributing to the progression of the research.

I have been working in Dr.Thomas Weimbs’ lab studying polycystic kidney disease using rats and mice as animal models. They are so cute! But I have learned that working with these animals is not a joke, it is actually a lot of responsibility. These animals’ lives are being sacrificed and that means you definitely do not want to mess up the experiment because their lives will have just been wasted. And believe me when I say they are really cute! And sometimes a little aggressive… In the lab, we try to make sure that everything is done in a way that inflicts the least amount of pain for these animals. But I still feel bad. Really bad for them. It definitely requires a lot of courage to work with these animals but the one thing I am glad about is gaining the experience and learning from it.

Throughout my three weeks in the lab, I have compiled a little list of tips that would have been very helpful to me if I was thinking about going into a research lab.

#1. Previous courses:

I have taken Bio lab and the introductory Bio courses but I never retained the information because I was just studying it for the finals. But this is a bad mindset. Learn the material to understand it fully, not just for the test. A lot of the stuff from those courses will need to be recalled in the lab but I found myself not being able to recall anything… which is just embarrassing and reflects badly on me.

#2. Note-Taking:

I have realized how important it is to be able to take neat and effective notes in the lab. My note taking skills are lacking but I want to improve upon them. Research is a very meticulous process and there are many details and data that need to be noted.

#3. Being proactive:

You will spend a lot of time at your postdoc’s side, learning from them, doing what they tell you, and sometimes they can not always be there to guide you… and in the lab there is a lot of downtime sometimes so it is up to you how you spend that time. You have to be proactive in finding productive things to do during those times. It is good to read papers on your research topic so you can become more familiar with it or even go over protocols.

“Can You Repeat That?”

“Can you repeat that?” is what I’ve been asking my mentor for the past three weeks. It is amazing how much I don’t know. This realization has come to me after beginning to work in a genetics lab within the chemical engineering department this summer. On multiple occasions, my chemical engineering professors have claimed chemical engineers are highly suited for nearly any position in stem because of our extremely versatile and rigorous curriculum. While I still believe this claim has merit, I think it is up for review with me as the case study. There is one aspect from the curriculum that I adhere to every day as an intern. That is: know it now, and know it alone. Prior to joining Professor Dey’s lab, I had basic knowledge of genetic processes acquired from obtaining an associate’s degree in biology. This knowledge served to stimulate my interests in the field, however it was far from sufficient to allow me intricate understanding of the methods used by professor Dey and his lab. I had a lot to learn, and I still do. For the past three weeks, I have worked on learning genetic processes, cellular processes, two new programming languages, DNA sequencing techniques, Dr. Dey’s novel technique for detecting double stranded DNA breaks (stay tuned for publications), DNA repair mechanisms, and much much more.

Starting out is the hardest part. The first week was the roughest because I needed to build a foundation of knowledge on which to build upon. Research is acquiring knowledge that has not yet been discovered. This aspect of research makes it quite exciting, but also difficult when starting out. It is difficult because previous knowledge in the field must be completely mastered so that previous knowledge can be applied to acquire new knowledge. This means countless hours reading papers published by scientists currently and previously in the field. I am new to regularly reading scientific papers and I have been previously spoiled by clearly written and edited textbooks. Every week I read at least three papers and provide summaries and insights that would help our research. While reading a paper, it is important to write down unknown words and obtain knowledge on them. The first paper I completed took me a few days. This is because every sentence had concepts and terminology I was unfamiliar with. It was a game of google searches and asking for help from my mentor.

These past few weeks I have learned how important networking is. The term networking seems like an artificial term which is why I think of networking as the human connection. It’s important to have the favor of people so they are willing to help. For example, I am in a new lab that is just starting out. We have limited funds and our lab is not set up yet. The graduate student, with the office next to ours, is working in a field similar to ours. He comes into our office regularly to store his food in the fridge and we would talk to him. One conversation we mentioned culturing cells in a pay per hour lab on campus. This graduate student has a cell culture station in his lab that is rarely used and he offered to let us use it, free of charge. This connection will allow my lab to conserve its resources and maybe offer collaboration between labs.

My research experience has just begun and I have a long way to go. If I can offer any advice to incoming researchers, it would be to humble yourself to learning. Starting out is rough and daunting, but perseverance rewards personal elevation and passion. An undergraduate curriculum is a passive experience; however, research requires one to take action. On your MARC, get set, go!